-Actinin Accumulation in Epithelial Cells Infected with Attaching and Effacing Gastrointestinal Pathogens Academic Article uri icon

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abstract

  • Fluorescent F-actin staining utilizing phalloidin, a highly toxic mushroom poison, is used as an indirect test to detect attaching and effacing (AE) bacteria. A study was done to determine if accumulation of alpha-actinin in infected tissue culture cells is a consistent feature and whether it corresponds with the AE response. Rearrangement of alpha-actinin was detected using immunofluorescence microscopy by incubation of infected cells with a murine monoclonal anti-alpha-actinin antibody. Foci of alpha-actinin-specific fluorescence corresponding to areas of bacterial adhesion were detected by transmission electron microscopy in HEp-2 and gastric KATO-III cells infected with only those bacterial strains that formed AE lesions. Therefore, this study shows that alpha-actinin accumulation is a consistent, specific manifestation of the AE phenotype and forms the basis for the development of a safe alternative test for detecting AE bacteria.

authors

  • Ismaili, A
  • Philpott, DJ
  • Dytoc, MT
  • Soni, R
  • Ratnam, Sam
  • Sherman, PM

publication date

  • November 1, 1995

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