Journal article
Diagnosis of Complex I Deficiency in Patients with Lactic Acidemia Using Skin Fibroblast Cultures
Abstract
The requirement for a rapid and easy method of preparing mitochondrial fractions from cultured skin fibroblasts led us to compare the results obtained from such a preparation with the more traditional methods of cellular fractionation. Values for NADH-cytochrome c reductase (rotenone sensitive) were compared for a series of three controls and nine patients with complex I (NADH-coenzyme Q reductase deficiency). Values obtained for deficient cell …
Authors
Pitkänen S; Raha S; Robinson BH
Journal
Molecular Genetics and Metabolism, Vol. 59, No. 2, pp. 134–137
Publisher
Elsevier
Publication Date
12 1996
DOI
10.1006/bmme.1996.0078
ISSN
1096-7192