Comet Assay in Cancer Chemoprevention Journal Articles

abstract

• The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.

authors

• Santoro, Raffaela
• Ferraiuolo, Maria
• Morgano, Gian Paolo
• Muti, Paola
• Strano, Sabrina

status

• published 

publication date

• 2016

has subject area

• 0399 Other Chemical Sciences (FoR)
• 0601 Biochemistry and Cell Biology (FoR)
• Cell Death (MeSH)
• Chemoprevention (MeSH)
• Comet Assay (MeSH)
• DNA Breaks, Double-Stranded (MeSH)
• DNA Breaks, Single-Stranded (MeSH)
• Developmental Biology (Science Metrix)
• Molecular Imaging (MeSH)
• Neoplasms (MeSH)
• Staining and Labeling (MeSH)

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Cell Death
• Chemoprevention
• Comet Assay
• Comet assay
• DNA Breaks, Double-Stranded
• DNA Breaks, Single-Stranded
• DNA damage
• DNA repair
• Double-strand breaks (DSB)
• Genotoxic stress
• Molecular Imaging
• Neoplasms
• Single-cell gel electrophoresis (SCGE)
• Single-strand breaks (SSB)
• Staining and Labeling

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-3191-0_9

PubMed ID

• 26608293

start page

• 99

end page

• 105

volume

• 1379