Activation of Mitogen-activated Protein Kinases During Human Lung Transplantation Academic Article uri icon

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  • BACKGROUND: Ischemia-reperfusion is one of the unavoidable steps in lung transplantation; it is associated with acute inflammatory responses and cell death. The intracellular signal transduction mechanisms of these events are largely unknown. We hypothesize that activation of mitogen-activated protein kinases (MAPKs) is one of the important signaling events during human lung transplantation. METHODS: Lung tissue biopsies were performed on 15 patients undergoing transplantation: after cold ischemic preservation; after warm ischemia (implantation); and after 1- or 2-hour reperfusion. The phosphorylation status of MAPK isoforms (ERK, p38-MAPK and JNK) was examined by Western blotting. RESULTS: Phosphorylation of ERK was dramatically increased during the first 2 hours of reperfusion. Phosphorylation of JNK also significantly increased at lower levels. In contrast, phosphorylation of p38 showed no significant changes. CONCLUSIONS: We speculate that the rapid and sustained activation of ERK and JNK during the early reperfusion period may contribute to acute inflammatory responses and cell death of lung grafts.


  • Sakiyama, Shoji
  • Hamilton, Julia
  • Han, Bing
  • Jiao, Yang
  • Shen-Tu, Grace
  • de Perrot, Marc
  • Keshavjee, Shafique
  • Liu, Mingyao

publication date

  • December 2005

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