Journal article
Synthesis of the herpes simplex virus type 1 trans-activator Vmw65 in insect cells using a baculovirus vector
Abstract
Vmw65, the Herpes Simplex Virus trans-activator of immediate-early genes, was expressed in insect cells using a recombinant baculovirus expression vector and partially purified. Insect cell-derived Vmw65 was shown to be indistinguishable from authentic Vmw65 present in purified HSV-1 virions based on electrophoretic mobility, immunoreactivity with a monoclonal antibody, and ability to interact with cellular factors to form a protein/DNA complex …
Authors
Capone JP; Werstuck G
Journal
Molecular and Cellular Biochemistry, Vol. 94, No. 1, pp. 45–52
Publisher
Springer Nature
Publication Date
April 1990
DOI
10.1007/bf00223561
ISSN
0300-8177