AbstractThe article contains sections titled:1.Introduction2.Cell Disruption3.Solid–Liquid Separation3.1.Conventional Filtration3.2.Membrane Filtration3.2.1.Promotion of Turbulence3.2.2.Application of Electric Field3.3.Centrifugation3.4.Coagulation and Flocculation4.Product Isolation4.1.Extraction4.2.Adsorption Including Ion Exchange4.2.1.Affinity Adsorption4.2.2.Ion‐Exchange Adsorption4.2.3.Quantitative Analysis4.2.4.Elution4.3.Precipitation4.3.1.Salting Out4.3.2.pH Adjustment4.3.3.Organic Solvents4.3.4.Temperature4.3.5.Polymers and Polyelectrolytes4.3.6.Affinity Precipitation4.4.Solubilization and Refolding4.5.Ultrafiltration5.Purification5.1.Chromatography5.1.1.Gel Filtration Chromatography5.1.2.Affinity Chromatography5.1.3.Reverse Phase and Hydrophobic Interaction Chromatography5.1.4.Quantitative Aspects5.2.Electrical Separations5.2.1.Electrophoresis5.2.2.Isoelectric Focusing and Isotachophoresis5.2.3.Electrodialysis5.2.4.Dielectrophoresis5.3.Magnetic Separations5.4.Membrane Chromatography6.Polishing6.1.Crystallization6.2.Drying and Freeze Drying6.3.Buffer Exchange6.4.Virus and Endotoxin Removal6.5.Removal of Aggregates7.New Bioseparation Strategies
