Unmasking Stemness and Immune Cell Markers in Brain Tumors with Immunohistochemistry

Immunohistochemistry (IHC) is a useful method to detect and visualize targeted proteins in tissues, with a high degree of specificity and clarity. Through the strategic use of chromogens and counterstains, it enables a contrast-rich and accurate evaluation of specific molecules, such as surface markers. Here, we outline a comprehensive IHC followed with counterstaining protocol for the positive selection of stem-like cells and immune cells in brain tumor tissue.