Computer calculation of radioimmunoassays

In order to develop a computer program to calculate radioimmunoassay data, the shape of the standard curve was investigated using orthogonal polynomials. Quadratic polynomials were found to fit the curves with a high degree of accuracy, and a computer program was developed using such a formula. Hormone levels are now routinely calculated by substitution into the polynomial. Statistical analysis of each curve permits immediate recognition of technical problems in the assay procedure.