Immunohistological localization of testosterone in the growing antler of the white-tailed deer (Odocoileus virginianus)

Using immunohistological methods (fluorescein and peroxidase labeled double antibody technique), the localization of testosterone in the antlers of white-tailed deer (Odocoileus virginianus) at the beginning of the period of accelerated calcification was examined. The most intense reaction for testosterone was found in the prochondral blastema layer and in the growing follicles of the velvet. The findings may indicate the importance of testosterone in bone matrix synthesis.