Cytotoxic lanostane-type triterpenes from the fruiting bodies of Ganoderma lucidum

A new lanostane-type triterpene, namely 3-oxo-5α-lanosta-7,9(11)-dien-24-oic acid methyl ester (2), and three known compounds including ganoderal A (1), ganoderiol B (3) and ganodermenonol (4) were isolated from the fruiting bodies of Ganoderma lucidum by silica gel column chromatography and Sephadex LH-20 column chromatography. Their structures were determined by extensive NMR data and mass spectral analysis. The in vitro cytotoxic activity of the isolated compounds against SK-Hep-1, HepG2, Hela and Hela/VCR cancer cell lines was assessed by using MTT assay. The IC₅₀ values of compound 1 were 43.09 ± 2.86, 42.31 ± 1.78 and 46.51 ± 1.95 μM in SK-Hep-1, HepG2 and Hela cells, respectively, after 48 h. The IC₅₀ values of compound 4 were 44.70 ± 2.32 and 41.33 ± 2.15 μM in Hela and Hela/VCR cells, respectively, after 48 h.