Journal article
High-throughput full-length single-cell RNA-seq automation
Abstract
Existing protocols for full-length single-cell RNA sequencing produce libraries of high complexity (thousands of distinct genes) with outstanding sensitivity and specificity of transcript quantification. These full-length libraries have the advantage of allowing probing of transcript isoforms, are informative regarding single-nucleotide polymorphisms and allow assembly of the VDJ region of the T- and B-cell-receptor sequences. Since full-length …
Authors
Mamanova L; Miao Z; Jinat A; Ellis P; Shirley L; Teichmann SA
Journal
Nature Protocols, Vol. 16, No. 6, pp. 2886–2915
Publisher
Springer Nature
Publication Date
June 2021
DOI
10.1038/s41596-021-00523-3
ISSN
1754-2189