Experts has a new look! Let us know what you think of the updates.

Provide feedback
Home
Scholarly Works
High-throughput full-length single-cell RNA-seq...
Journal article

High-throughput full-length single-cell RNA-seq automation

Abstract

Existing protocols for full-length single-cell RNA sequencing produce libraries of high complexity (thousands of distinct genes) with outstanding sensitivity and specificity of transcript quantification. These full-length libraries have the advantage of allowing probing of transcript isoforms, are informative regarding single-nucleotide polymorphisms and allow assembly of the VDJ region of the T- and B-cell-receptor sequences. Since full-length …

Authors

Mamanova L; Miao Z; Jinat A; Ellis P; Shirley L; Teichmann SA

Journal

Nature Protocols, Vol. 16, No. 6, pp. 2886–2915

Publisher

Springer Nature

Publication Date

June 2021

DOI

10.1038/s41596-021-00523-3

ISSN

1754-2189