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Targeted amplification-based whole genome...
Journal article

Targeted amplification-based whole genome sequencing of Monkeypox virus in clinical specimens

Abstract

IMPORTANCE: We present a protocol to efficiently sequence genomes of the MPXV-causing mpox. This enables researchers and public health agencies to acquire high-quality genomic data using a rapid and cost-effective approach. Genomic data can be used to conduct surveillance and investigate mpox outbreaks. We present 91 mpox genomes that show the diversity of the 2022 mpox outbreak in Ontario, Canada.

Authors

Isabel S; Eshaghi A; Duvvuri VR; Gubbay JB; Cronin K; Li A; Hasso M; Clark ST; Hopkins JP; Patel SN

Journal

Microbiology Spectrum, Vol. 12, No. 1, pp. e02979–e02923

Publisher

American Society for Microbiology

Publication Date

January 11, 2024

DOI

10.1128/spectrum.02979-23

ISSN

2165-0497