Preprint
PAM-Flexible Genome Editing with an Engineered Chimeric Cas9
Abstract
CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting domain of SpRY, a broad-targeting Cas9 possessing an NRN > NYN PAM preference, with the N-terminus of Sc++, a Cas9 with simultaneously broad, efficient, and accurate NNG editing capabilities, to generate a …
Authors
Koseki S; Hong L; Yudistyra V; Stan T; Tysinger E; Silverstein R; Kramme C; Amrani N; Savic N; Pacesa M
Pagination
pp. rs.3.rs-2625838
DOI
10.21203/rs.3.rs-2625838/v1
Preprint server
Research Square