The purpose of this study is to examine the mediating role of symptoms of stress on the relationship between perceived access to training and job satisfaction. The changing nature of work (i.e. workplace and job complexity) has implications for stress and job satisfaction outcomes. Concerns about stress and job satisfaction levels and their link to performance have created interest in training interventions to improve the adequacy of knowledge and skills. A cross-sectional questionnaire survey of 1396 nurses was collected from three large teaching hospitals. The positive relationship between employees’ perceived access to training and job satisfaction is increased by the partial mediation of symptoms of stress. Based on the evidence of this study, we recommend human resource staff and managers focus on training as a factor enhancing job satisfaction and mitigating stress.