901 — Electrode process of glutamate dehydrogenase in differential pulse polarography

The anodic and cathodic responses of sulfhydryl groups in glutamate dehydrogenase (GDH) were studied using the d.p.p. method. The current peaks were detected at the potentials Up,anod and Up,cath of approx. −1.45 V and −1.50 V, respectively, versus s.c.e. in phosphate buffer, pH 7. Both the cathodic and the anodic responses of GDH showed autoinhibiting diffusion-controlled adsorption effects. Both peak potentials Up,anod and Up,cath are shifted …