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Comparative Analysis of Metagenomic (Amplicon and...
Journal article

Comparative Analysis of Metagenomic (Amplicon and Shotgun) DNA Sequencing to Characterize Microbial Communities in Household On-Site Wastewater Treatment Systems

Abstract

The performance of on-site wastewater treatment systems (OWTSs) can be improved by altering digester design and by manipulating environmental variables that affect microbial community composition. Community composition can potentially be assessed using high-throughput DNA sequencing, but the two most common methods of community DNA sequencing (16S and shotgun sequencing) generally yield different taxonomic identification profiles and can …

Authors

de Vries J; Saleem F; Li E; Chan AWY; Naphtali J; Naphtali P; Paschos A; Schellhorn HE

Journal

Water, Vol. 15, No. 2,

Publisher

MDPI

DOI

10.3390/w15020271

ISSN

2073-4441