Journal article
Properties of common carp gonadotropin I and gonadotropin II
Abstract
Two gonadotropins, GtH I and GtH II, were extracted with 35% ethanol-10% ammonium acetate, pH 6.1, from female common carp pituitary glands and purified by ion-exchange chromatography on a DE-52 column followed by gel filtration on a Sephadex G-75 column. Molecular weights of GtH I and GtH II as determined by SDS-PAGE were 45,000 and 35,000, respectively. Both GtHs dissociate into two subunits following reduction with beta-mercaptoethanol. …
Authors
Van Der Kraak G; Suzuki K; Peter RE; Itoh H; Kawauchi H
Journal
General and Comparative Endocrinology, Vol. 85, No. 2, pp. 217–229
Publisher
Elsevier
Publication Date
February 1992
DOI
10.1016/0016-6480(92)90005-5
ISSN
0016-6480