Journal article
Ultrasonic and Densimetric Characterization of the Association of Cyclic AMP with the cAMP-Binding Domain of the Exchange Protein EPAC1
Abstract
We employed a combination of densimetric and ultrasonic velocimetric techniques to characterize the volumetric properties of the association of the cAMP-binding domain (CBD) of EPAC1 with cAMP at 25 °C in a pH 7.6 buffer. The binding of cAMP to the CBD of EPAC1 is accompanied by changes in volume, ΔV, and adiabatic compressibility, ΔKS, of -59 ± 4 cm(3) mol(-1) and (34 ± 9) × 10(-4) cm(3) mol(-1) bar(-1), respectively. We use these volumetric …
Authors
Son I; Selvaratnam R; Dubins DN; Melacini G; Chalikian TV
Journal
The Journal of Physical Chemistry B, Vol. 117, No. 37, pp. 10779–10784
Publisher
American Chemical Society (ACS)
Publication Date
September 19, 2013
DOI
10.1021/jp406451p
ISSN
1520-6106