Differential staining of the X-chromosome during meiosis of orthoptera by a silver impregnation procedure Academic Article uri icon

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abstract

  • Differential staining of the X chromosome of Orthoptera, which normally cannot be distinguished from the autosomes during the more condensed stages of meiosis, has been achieved by a silver impregnation procedure involving a pretreatment with 2 x SSC at 60 degrees C. The results suggest that the saline citrate solution preferentially extracts proteins from the X chromosome. This method may be useful for distinguishing chromosome regions composed of facultative heterochromatin in Orthoptera.

authors

  • Fernandez-Piqueras, Jos
  • Campos, Ana Regina
  • Casta�o, Carlos Sentis
  • Garcia, Enrique Rojo

publication date

  • August 1982