Journal article
Changes in fluid secretion rate alter net transepithelial transport of MRP2 and P‐glycoprotein substrates in Malpighian tubules of Drosophila melanogaster
Abstract
The effects of stimulants of fluid secretion on net transepithelial transport of the MRP2 substrate Texas Red and the p-glycoprotein substrate daunorubicin were examined in Malpighian tubules of Drosophila melanogaster. Fluid secretion rates were determined using the Ramsay assay and secreted fluid concentrations of Texas Red and daunorubicin were determined using a microfluorometric technique. Nanoliter droplets of secreted fluid were …
Authors
O'Donnell MJ; Leader JP
Journal
Archives of Insect Biochemistry and Physiology, Vol. 63, No. 3, pp. 123–134
Publisher
Wiley
Publication Date
November 2006
DOI
10.1002/arch.20148
ISSN
0739-4462
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
ATP Binding Cassette Transporter, Subfamily BAnimalsBiological TransportBody FluidsCyclic AMPDrosophila melanogasterEpitheliumFlow CytometryMalpighian TubulesMembrane Transport ProteinsMicroscopy, ConfocalMultidrug Resistance-Associated Protein 2Multidrug Resistance-Associated ProteinsRegression AnalysisTyramineXanthenes