General transduction in Rhizobium meliloti Journal Articles

abstract

• General transduction by phage phi M12 in Rhizobium meliloti SU47 and its derivatives is described. Cotransduction and selection for Tn5 insertions which are closely linked to specific loci were demonstrated. A derivative of SU47 carrying the recA::Tn5 allele of R. meliloti 102F34 could be transduced for plasmid R68.45 but not for chromosomally located alleles. Phage phi M12 is morphologically similar to Escherichia coli phage T4, and restriction endonuclease analysis indicated that the phage DNA was ca. 160 kilobases in size.

authors

• Finan, Turlough Mary
• Hartweig, E
• LeMieux, K
• Bergman, K
• Walker, GC
• Signer, ER

status

• published 

publication date

• July 1984

has subject area

• 06 Biological Sciences (FoR)
• 07 Agricultural and Veterinary Sciences (FoR)
• 11 Medical and Health Sciences (FoR)
• Bacteriophages (MeSH)
• DNA Transposable Elements (MeSH)
• Escherichia coli (MeSH)
• Genotype (MeSH)
• Microbiology (Science Metrix)
• Plasmids (MeSH)
• Rhizobium (MeSH)
• Transduction, Genetic (MeSH)

published in

• Journal of Bacteriology  Journal

keywords

• Bacteriophages
• DNA Transposable Elements
• Escherichia coli
• Genotype
• Plasmids
• Rhizobium
• Transduction, Genetic

Digital Object Identifier (DOI)

• 10.1128/jb.159.1.120-124.1984

start page

• 120

end page

• 124

volume

• 159

issue

• 1