Host-dependent transposon Tn5-mediated streptomycin resistance Journal Articles

abstract

• Transposon Tn5 encodes streptomycin resistance in addition to kanamycin-neomycin resistance. This resistance was not detectable in Escherichia coli but was efficiently expressed in Rhizobium meliloti and certain other strains. By analysis of cloned Tn5 restriction endonuclease fragments, the streptomycin resistance (str) gene was located in the right-hand side of the central region as the transposon is conventionally drawn. Transcription of str appeared to originate at pL, the promoter for the neo gene (neomycin phosphotransferase type II). Expression of streptomycin resistance in E. coli was obtained after cloning of the neo-str region downstream of a strong E. coli promoter. A construct in which PL was deleted also showed differential expression of streptomycin resistance.

authors

• De Vos, GF
• Finan, Turlough Mary
• Signer, ER
• Walker, GC

status

• published 

publication date

• July 1984

has subject area

• 06 Biological Sciences (FoR)
• 07 Agricultural and Veterinary Sciences (FoR)
• 11 Medical and Health Sciences (FoR)
• Cloning, Molecular (MeSH)
• DNA Restriction Enzymes (MeSH)
• DNA Transposable Elements (MeSH)
• Drug Resistance, Microbial (MeSH)
• Escherichia coli (MeSH)
• Klebsiella pneumoniae (MeSH)
• Microbiology (Science Metrix)
• Plasmids (MeSH)
• Rhizobium (MeSH)
• Species Specificity (MeSH)
• Streptomycin (MeSH)
• Transcription, Genetic (MeSH)

published in

• Journal of Bacteriology  Journal

keywords

• Cloning, Molecular
• DNA Restriction Enzymes
• DNA Transposable Elements
• Drug Resistance, Microbial
• Escherichia coli
• Klebsiella pneumoniae
• Plasmids
• Rhizobium
• Species Specificity
• Streptomycin
• Transcription, Genetic

Digital Object Identifier (DOI)

• 10.1128/jb.159.1.395-399.1984

start page

• 395

end page

• 399

volume

• 159

issue

• 1