Journal article
Cloning and characterization of the pyruvate carboxylase from Sinorhizobium meliloti Rm1021
Abstract
Abstract. The gene encoding pyruvate carboxylase (pyc) was isolated from a Sinorhizobium meliloti Rm1021 cosmid bank by complementation of a Rhizobium tropicipyc mutant. PYC-negative mutants of S. meliloti Rm1021 were isolated by transposon mutagenesis and were unable to grow with glucose or pyruvate as sole carbon sources, but were symbiotically competent in combination with alfalfa plants. PYC activity assays, pyc::lacZ gene fusion studies …
Authors
Dunn MF; Araíza G; Finan TM
Journal
Archives of Microbiology, Vol. 176, No. 5, pp. 355–363
Publisher
Springer Nature
Publication Date
November 2001
DOI
10.1007/s002030100336
ISSN
0302-8933