Journal article
Transduction of the CHOaprt gene into mouse L cells using an adeno-5/APRT recombinant virus
Abstract
An adenovirus-5 recombinant virus Adapt1 carrying the Chinese hamster ovary (CHO) adenine phosphoribosyltransferase (aprt) gene was constructed by insertion of a 2.5-kb fragment containing the complete CHOaprt structural gene linked to a Moloney murine sarcoma virus (MSV) promoter into the E3 region of adenovirus-5. The CHOaprt gene was in the opposite orientation to the adenovirus E3 promoter. Mouse Lapt− tk− (LAT) cells expressed the CHOaprt …
Authors
Konan V; Sahota A; Graham FL; Taylor MW
Journal
Somatic Cell and Molecular Genetics, Vol. 17, No. 4, pp. 359–368
Publisher
Springer Nature
Publication Date
July 1991
DOI
10.1007/bf01233061
ISSN
0740-7750