Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites Academic Article uri icon

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  • Coronavirus disease (COVID-19) is a new and highly contagious disease posing a threat to global public health and wreaking havoc around the world. It's caused by the Coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2). In the current pandemic situation, rapid and accurate SARS-CoV-2 diagnosis on a large scale is critical for early-stage diagnosis. Early detection and monitoring of viral infections can aid in controlling and preventing infection in large groups of people. Accordingly, we developed a sensitive and high-throughput sandwich electrochemiluminescence immunosensor based on antigen detection for COVID-19 diagnosis (the spike protein of SARS-CoV-2). For the spike protein of SARS-CoV-2, the ECL biosensor had a linear range of 10 ng mL-1 to 10 µg mL-1 with a limit of detection of 1.93 ng mL-1. The sandwich ECL immunosensor could be used in early clinical diagnosis due to its excellent recovery in detecting SARS-CoV-2, rapid analysis (90 min), and ease of use.


  • Hosseini, Morteza
  • Sobhanie, Ebtesam
  • Salehnia, Foad
  • Xu, Guobao
  • Rabbani, Hodjattallah
  • Naghavi Sheikholeslami, Mahsa
  • Firoozbakhtian, Ali
  • Sadeghi, Niloufar
  • Hossein Farajollah, Mohammad
  • Reza Ganjali, Mohammad
  • Vosough, Houman

publication date

  • October 2022