Journal article
2‘-Hydroxypyridoxol, a Biosynthetic Precursor of Vitamins B6 and B1 in Yeast
Abstract
In separate experiments cultures of the yeast Saccharomyces cerevisiae ATTC 7752 were grown in the presence of [5',5'-2H2]- or of [2',2',5',5'-2H4]-3-hydroxy-2,4,5-tri(hydroxymethyl)pyridine (i.e., 2'-hydroxypyridoxol). The 2H NMR spectra of the samples of pyridoxamine dihydrochloride and of thiamin chloride hydrochloride that were isolated from the two experiments showed the presence of deuterium at the corresponding sites. Entry of deuterium …
Authors
Zeidler J; Ullah N; Gupta RN; Pauloski RM; Sayer BG; Spenser ID
Journal
Journal of the American Chemical Society, Vol. 124, No. 17, pp. 4542–4543
Publisher
American Chemical Society (ACS)
Publication Date
May 1, 2002
DOI
10.1021/ja012708z
ISSN
0002-7863