Induction of rod versus cone photoreceptor-specific progenitors from retinal precursor cells Journal Articles uri icon

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abstract

  • During development, multipotent progenitors undergo temporally-restricted differentiation into post-mitotic retinal cells; however, the mechanisms of progenitor division that occurs during retinogenesis remain controversial. Using clonal analyses (lineage tracing and single cell cultures), we identify rod versus cone lineage-specific progenitors derived from both adult retinal stem cells and embryonic neural retinal precursors. Taurine and retinoic acid are shown to act in an instructive and lineage-restricted manner early in the progenitor lineage hierarchy to produce rod-restricted progenitors from stem cell progeny. We also identify an instructive, but lineage-independent, mechanism for the specification of cone-restricted progenitors through the suppression of multiple differentiation signaling pathways. These data indicate that exogenous signals play critical roles in directing lineage decisions and resulting in fate-restricted rod or cone photoreceptor progenitors in culture. Additional factors may be involved in governing photoreceptor fates in vivo.

authors

  • Khalili, Saeed
  • Ballios, Brian G
  • Belair-Hickey, Justin
  • Donaldson, Laura
  • Liu, Jeff
  • Coles, Brenda LK
  • Grisé, Kenneth N
  • Baakdhah, Tahani
  • Bader, Gary D
  • Wallace, Valerie A
  • Bernier, Gilbert
  • Shoichet, Molly S
  • van der Kooy, Derek

publication date

  • December 2018