The influence of body mass index on islet isolation outcome and function

Objective To investigate the difference between the donors body mass index(BMI) and the islet isolation yield and function of islet. Methods The 93 cases of pancreas were collected for our study. According to the obesity classification standard in Asian, the donors were divided into three groups, including the normal group (BMI: 18.50-22.99 kg/m 2 ), the overweight group (BMI: 23.00-24.99 kg/m 2 ) and the obese group (BMI≥25.00 kg/m 2 ). The method from Ricordi was used for digestion, and islets were purified by continuous density gradient method. The islets were identified by staining with the Dithizone (DTZ), the islets were analyzed for cell viability, and the insulin secretion ability of islets was determined by GSIS test. Results The islets yield (IEQ) in BMI≥25.00 kg/m 2 group was more than other two groups ( F =3.05, P <0.05). Pearson analysis showed that the level of donor BMI was positively correlated with islet function ( r =0.37, P =0.01). Conclusion The higher level of donor BMI may be associated with more islets yield and better islet function in certain range of isolation BMI.