A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies Academic Article uri icon

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abstract

  • The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.

authors

  • Russell, Gabriel A
  • Peng, Garrie
  • Faubert, Cynthia
  • Verdu, Elena
  • Hapfelmeier, Siegfried
  • King, Irah L

publication date

  • December 2021