Journal article
Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome
Abstract
The main viral protease (3CLpro) is indispensable for SARS-CoV-2 replication. We delineate the human protein substrate landscape of 3CLpro by TAILS substrate-targeted N-terminomics. We identify more than 100 substrates in human lung and kidney cells supported by analyses of SARS-CoV-2-infected cells. Enzyme kinetics and molecular docking simulations of 3CLpro engaging substrates reveal how noncanonical cleavage sites, which diverge from …
Authors
Pablos I; Machado Y; de Jesus HCR; Mohamud Y; Kappelhoff R; Lindskog C; Vlok M; Bell PA; Butler GS; Grin PM
Journal
Cell Reports, Vol. 37, No. 4,
Publisher
Elsevier
Publication Date
October 2021
DOI
10.1016/j.celrep.2021.109892
ISSN
2639-1856