Distinct patterns of within-host virus populations between two subgroups of human respiratory syncytial virus Academic Article uri icon

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abstract

  • AbstractHuman respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infection in young children globally, but little is known about within-host RSV diversity. Here, we characterised within-host RSV populations using deep-sequencing data from 319 nasopharyngeal swabs collected during 2017–2020. RSV-B had lower consensus diversity than RSV-A at the population level, while exhibiting greater within-host diversity. Two RSV-B consensus sequences had an amino acid alteration (K68N) in the fusion (F) protein, which has been associated with reduced susceptibility to nirsevimab (MEDI8897), a novel RSV monoclonal antibody under development. In addition, several minor variants were identified in the antigenic sites of the F protein, one of which may confer resistance to palivizumab, the only licensed RSV monoclonal antibody. The differences in within-host virus populations emphasise the importance of monitoring for vaccine efficacy and may help to explain the different prevalences of monoclonal antibody-escape mutants between the two subgroups.

authors

  • Lin, Gu-Lung
  • Drysdale, Simon B
  • Snape, Matthew D
  • O’Connor, Daniel
  • Brown, Anthony
  • MacIntyre-Cockett, George
  • Mellado-Gomez, Esther
  • de Cesare, Mariateresa
  • Bonsall, David
  • Ansari, M Azim
  • Öner, Deniz
  • Aerssens, Jeroen
  • Butler, Christopher
  • Bont, Louis
  • Openshaw, Peter
  • Martinón-Torres, Federico
  • Nair, Harish
  • Bowden, Rory
  • Campbell, Harry
  • Cunningham, Steve
  • Bogaert, Debby
  • Beutels, Philippe
  • Wildenbeest, Joanne
  • Clutterbuck, Elizabeth
  • McGinley, Joseph
  • Thwaites, Ryan
  • Wiseman, Dexter
  • Gómez-Carballa, Alberto
  • Rodriguez-Tenreiro, Carmen
  • Rivero-Calle, Irene
  • Dacosta-Urbieta, Ana
  • Heikkinen, Terho
  • Meijer, Adam
  • Fischer, Thea Kølsen
  • van den Berge, Maarten
  • Giaquinto, Carlo
  • Abram, Michael
  • Dormitzer, Philip
  • Stoszek, Sonia
  • Gallichan, Scott
  • Rosen, Brian
  • Molero, Eva
  • Machin, Nuria
  • Spadetto, Martina
  • Golubchik, Tanya
  • Pollard, Andrew J

publication date

  • December 2021