Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis Academic Article uri icon

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abstract

  • The monitoring of epidemicPseudomonas aeruginosais important for cystic fibrosis (CF) infection control. The prairie epidemic strain (PES) is common in western Canadian CF clinics. Using whole-genome sequencing, we identified a novel genomic island and developed a PCR assay for PES. Against a collection of 186P. aeruginosaisolates, the assay had 98% sensitivity and 100% specificity.

authors

  • Workentine, M
  • Poonja, A
  • Waddell, B
  • Duong, J
  • Storey, DG
  • Gregson, D
  • Somayaji, R
  • Rabin, HR
  • Surette, Michael
  • Parkins, MD

publication date

  • February 2016