abstract
- The objective of the present work was to determine the optimum dietary protein level for juvenile mullets. Five isocaloric diets were formulated to contain increasing levels (300, 350, 400, 450 and 500 g kg⁻¹) of crude protein (CP) corresponding to 18.7 MJ metabolizable energy kg⁻¹. All diets were tested in triplicate. Each experimental unit was composed of a 50 L tank with 50 juveniles (mean ± SE initial weight and length equal to 1.17 ± 0.02 g and 4.34 ± 0.03 cm respectively). Diets were offered five times a day until apparent satiation for 35 days. No significant difference (P>0.05) was observed in survival rate, feed efficiency and body composition between treatments. However, weight gain, feed consumption and specific growth rate were higher in fish fed the 350 g kg⁻¹ CP level than those fed the highest protein content diet (500 g kg⁻¹ CP). The amount of postprandial ammonia excreted by mullet was linearly related to protein intake. Intestinal tryptic activity was inversely proportional to the percentage of dietary CP. It is likely that diets containing <350 g kg⁻¹ CP will be needed for on-growing mullet, especially when reared in ponds with abundant natural food.