Effect of adipose derived nucleated cell fractions with chitosan biodegradable film on wound healing in rats

Wound healing is the interaction of a complex event in cellular and biochemical actions ending up the reestablishment of structural and functional integrity of injured tissues. In this study the effect of combination of adipose derived nucleated cell fractions (ADNCs) and chitosan film was evaluated on full thickness wounds. For excisional wound healing model twenty-four male white Wistar rats were randomized into four groups of 6 rats each: control group (CG) including creation of wounds and no treatment, ADNC group including creation of wounds and application of 1mL ADNCs, chitosan group (CHIT) including dressing the wound with chitosan and CHIT/ADNC group including application of 1mL ADNCs and dressing the wound with chitosan. Wound size was measured on 6, 9, 12, 15, 18 and 21 days after surgery. For incisional wound healing model twenty-four healthy male Wistar rats were randomized into four groups of 6 rats each the same way in excisional wound model. Reduction in wound area, hydroxyproline contents and biomechanical parameters indicated that there was significant difference (P>0.05) between CHIT/ADNC group and other groups. Biomechanical testing was performed 9 days after surgery in incisional model. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was significant difference (P>0.05) between CHIT/ADNC group and other groups. ADNCs can provide a readily accessible source of cells in large quantities that contribute to wound healing in the emerging field of regenerative medicine especially where a traumatic injury is dealt with. ADNCs with chitosan have beneficial effects on wounds repair and could be suggested for treating various types of wounds in animals and human being.