Chapter
Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells
Abstract
It has been recently identified that cytokines and BMP-4 promote hematopoiesis from human embryonic stem cells (hESC) and that, before hematopoietic commitment, a rare subpopulation of cells lacking CD45, but expressing PECAM-1, Flk-1, and VE-cadherin (hereinafter termed CD45negPFV precursors), are exclusively responsible for hematopoietic cell fate on cytokine stimulation. Efficient strategies to stably transduce these hematopoietic precursors …
Authors
Menendez P; Wang L; Cerdan C; Bhatia M
Book title
Human Embryonic Stem Cell Protocols
Volume
331
Pagination
pp. 201-220
Publisher
Springer Nature
Publication Date
2006
DOI
10.1385/1-59745-046-4:201
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Antigens, CDCadherinsCell Culture TechniquesCell DifferentiationCell SeparationFlow CytometryGenetic TherapyHematopoietic Stem CellsHumansLeukocyte Common AntigensPlatelet Endothelial Cell Adhesion Molecule-1Pluripotent Stem CellsRetroviridaeTransduction, GeneticVascular Endothelial Growth Factor Receptor-2