Journal article
Engineering Micrometer‐Sized DNA Tracks for High‐Speed DNA Synthesis and Biosensing
Abstract
Abstract φ 29 DNA polymerase (Pol φ 29) is capable of synthesizing long‐chain single‐stranded (ss) DNA molecules by copying the sequence of a small ss circular DNA template (ssCDT) in a process known as rolling circle amplification (RCA). The use of a ssCDT in RCA, however, comes with a key drawback: the rate of DNA synthesis is significantly reduced. We hypothesize that this issue can be overcome using a very long linear ssDNA template with a …
Authors
Wang L; Song K; Qu Y; Chang Y; Li Z; Dong C; Liu M; Brennan JD; Li Y
Journal
Angewandte Chemie, Vol. 132, No. 51, pp. 23147–23151
Publisher
Wiley
Publication Date
December 14, 2020
DOI
10.1002/ange.202010693
ISSN
0044-8249