Journal article
Proteomic responses in the gills of fathead minnows (Pimephales promelas, Rafinesque, 1820) after 6 months and 2 years of continuous exposure to environmentally relevant dietary 226Ra
Abstract
PURPOSE: To investigate changes in the gill proteome of fathead minnows continually fed an environmentally relevant dietary dose of (226)Ra for 2 years.
METHODS: The fish were fed a commercial diet containing 10 mBq-10 Bq (226)Ra g(-1). After 6 months and 2 years the gill proteome was analyzed by two-dimensional electrophoresis (2-DE). Protein spots which exhibited a significant change were identified using mass spectrometry.
Authors
Smith RW; Wang J; Mothersill CE; Lee LEJ; Seymour CB
Journal
International Journal of Radiation Biology, Vol. 91, No. 3, pp. 248–256
Publisher
Taylor & Francis
Publication Date
March 4, 2015
DOI
10.3109/09553002.2014.988894
ISSN
0955-3002
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Animal FeedAnimalsApolipoprotein A-ICyprinidaeElectrophoresis, Gel, Two-DimensionalFemaleFish ProteinsFood Contamination, RadioactiveGillsGlyceraldehyde-3-Phosphate DehydrogenasesMalate DehydrogenaseMaleProteomeProteomicsRadiation ToleranceRadiobiologyRadiumReactive Oxygen SpeciesSpectrometry, Mass, Electrospray IonizationTime Factors