Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants Academic Article uri icon

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abstract

  • Abstract Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. Clinical Trials Registration. NCT03627572 and NCT03756766.

authors

  • Lin, Gu-Lung
  • Golubchik, Tanya
  • Drysdale, Simon
  • O’Connor, Daniel
  • Jefferies, Kimberley
  • Brown, Anthony
  • de Cesare, Mariateresa
  • Bonsall, David
  • Ansari, M Azim
  • Aerssens, Jeroen
  • Bont, Louis
  • Openshaw, Peter
  • Martinón-Torres, Federico
  • Bowden, Rory
  • Pollard, Andrew J
  • Nair, Harish
  • Campbell, Harry
  • Cunningham, Steve
  • Beutels, Philippe
  • Bont, Louis
  • Wildenbeest, Joanne
  • Pollard, Andrew
  • Butler, Christopher
  • Snape, Matthew
  • Drysdale, Simon
  • Lin, Gu-Lung
  • O’Connor, Daniel
  • Clutterbuck, Elizabeth
  • Jefferies, Kimberley
  • McGinley, Joseph
  • Openshaw, Peter
  • Thwaites, Ryan
  • Wiseman, Dexter
  • Martinon-Torres, Federico
  • Gómez-Carballa, Alberto
  • Heikkinen, Terho
  • Meijer, Adam
  • Fischer, Thea Kølsen
  • van den Berge, Maarten
  • Giaquinto, Carlo
  • Abram, Michael
  • Swanson, Kena
  • Leach, Amanda
  • Demont, Clarisse
  • Gallichan, Scott
  • Aerssens, Jeroen
  • Öner, Deniz
  • Rosen, Brian
  • Molero, Eva
  • Nair, Harish
  • Campbell, Harry
  • Cunningham, Steve
  • Beutels, Philippe
  • Bont, Louis
  • Wildenbeest, Joanne
  • Pollard, Andrew
  • Butler, Christopher
  • Snape, Matthew
  • Drysdale, Simon
  • Lin, Gu-Lung
  • O’Connor, Daniel
  • Clutterbuck, Elizabeth
  • Jefferies, Kimberley
  • McGinley, Joseph
  • Openshaw, Peter
  • Thwaites, Ryan
  • Wiseman, Dexter
  • Martinon-Torres, Federico
  • Gómez-Carballa, Alberto
  • Heikkinen, Terho
  • Meijer, Adam
  • Fischer, Thea Kølsen
  • van den Berge, Maarten
  • Giaquinto, Carlo
  • Abram, Michael
  • Swanson, Kena
  • Leach, Amanda
  • Demont, Clarisse
  • Gallichan, Scott
  • Aerssens, Jeroen
  • Öner, Deniz
  • Rosen, Brian
  • Molero, Eva

publication date

  • October 7, 2020