Calcium protects pituitary basic fibroblast growth factors from limited proteolysis by co-purifying proteases Academic Article uri icon

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abstract

  • Extracts from bovine pituitaries and other tissues contained basic fibroblast growth factor-like peptides of 22-26 kda, co-fractionating with smaller, 16-20 kda bFGFs. Heparin-bound, 22-26 kda bFGFs were converted to smaller, heparin-binding forms by tryptic proteolysis. In solution, 22-26 kda bFGFs were converted to smaller, heparin-binding forms by an activity present in pituitary extracts. Calcium protected higher molecular weight pituitary bFGFs from truncation by the endogenous activity, which was not acid-activated, co-purified with bFGF during heparin-sepharose chromatography, remained operant at high salt concentrations and was inhibited by phenylmethan-sulfonyl fluoride.

authors

  • Doble, Bradley
  • Fandrich, Robert R
  • Liu, Lei
  • Padua, Raymond R
  • Kardami, Elissavet

publication date

  • December 1990