Journal article
Structural Effects of a Covalent Linkage Between Antithrombin and Heparin: Covalent N-Terminus Attachment of Heparin Enhances the Maintenance of Antithrombin's Activated State
Abstract
We have produced a molecule comprising of permanently-activated covalently linked antithrombin and heparin (ATH). This study was designed to elucidate the covalent linkage point(s) for heparin on antithrombin and conformational properties of the ATH molecule. ATH was produced using Schiff base/Amadori rearrangement by incubating antithrombin with unfractionated heparin for 14 d at 40 degrees C. ATH was then digested using Proteinase K, and the …
Authors
Mewhort-Buist TA; Junop M; Berry LR; Chindemi P; Chan AKC
Journal
The Journal of Biochemistry, Vol. 140, No. 2, pp. 175–184
Publisher
Oxford University Press (OUP)
Publication Date
August 1, 2006
DOI
10.1093/jb/mvj139
ISSN
0021-924X