Diagnosis Of Chlamydia Trachomatis Urethral Infection In Symptomatic And Asymptomatic Men By Testing First-Void Urine In A Ligase Chain Reaction Assay Journal Articles uri icon

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  • A multicenter study compared ligase chain reaction (LCR) of Chlamydia trachomatis plasmid DNA with culture of urethral swab specimens from 542 men (study A); a second study (B) compared LCR of first-void urine (FVU) with urethral swab cultures from 1043 men. Discordant results were resolved with direct fluorescent antibody staining of sediments from the FVU or urethral culture specimen and with a second LCR directed against a fragment of the major outer membrane protein gene. Test performance was calculated on the basis of an expanded reference standard. The LCR plasmid assay had a sensitivity of 98.0% in study A and 93.5% in study B; specificity was 99.8%-100%. The sensitivity of culturing urethral swabs from all study sites was 68.2% (range by sites, 40.0%-84.6%). The presence or absence of urethral symptoms did not alter the results. Use of this LCR test should allow more meaningful investigation and treatment of C. trachomatis infections in men.


  • Chernesky, Max
  • Lee, H
  • Schachter, J
  • Burczak, JD
  • Stamm, WE
  • Mccormack, WM
  • Quinn, TC

publication date

  • November 1, 1994