Journal article
Conformational Changes in Apolipoprotein B Modulate Intracellular Assembly and Degradation of ApoB-Containing Lipoprotein Particles in HepG2 Cells
Abstract
The linkage between the conformation of apolipoprotein B100 (apoB) and the intracellular assembly and degradation of apoB-containing lipoproteins was investigated in the present study. Disruption of disulfide bond formation in newly synthesized apoB molecules through the use of the reducing agent DTT resulted in a decrease in the secretion of apoB-containing lipoproteins from HepG2 cells compared with control cells. The synthesis of total apoB …
Authors
Macri J; Adeli K
Journal
Arteriosclerosis Thrombosis and Vascular Biology, Vol. 17, No. 11, pp. 2982–2994
Publisher
Wolters Kluwer
Publication Date
November 1997
DOI
10.1161/01.atv.17.11.2982
ISSN
1079-5642
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AnimalsApolipoprotein B-100Apolipoproteins BCarcinoma, HepatocellularCystineDithiothreitolGlycosylationHumansLeupeptinsLipoproteinsLipoproteins, HDLLipoproteins, LDLLipoproteins, VLDLLiverLiver NeoplasmsMicrosomes, LiverMolecular WeightNeoplasm ProteinsProtease InhibitorsProtein ConformationProtein DenaturationProtein FoldingProtein Processing, Post-TranslationalSulfhydryl ReagentsTumor Cells, CulturedTunicamycin