Objective: To quantitatively investigate the relationship between a known antigen encoded by adenoviral vector(Ad SIINFEKL-Luc) and the induced immune response. Methods: Murine bone marrow-derived dendritic cells(DC) were infected by Ad SIINFEKL-Luc encoding a fusion protein consisting of chicken ovalbumin MHC class I peptide SIINFEKL(OVAp257-264) and luciferase in different MOI and the expression levels of luciferase(Luc) in DC were quantitatively checked. C57BL/6 mice were subcutaneously immunized with the Ad SIINFEKL-Luc-infected DC, and then the killing activity of antigen-specific CTL in spleen were analyzed by in vivo CTL assay and intracellular cytokine staining(ICS) assay for IFN-γ produced by antigen-specific CD8+ T cells were performed. Results: A dose-dependent relationship between the levels of Luc expression and doses of Ad SIINFEKL-Luc was observed, and the expression of Luc could be still detected in DC 4 d after Ad SIINFEKL-Luc infection. One week after the subcutaneous immunization of C57BL/6 mice with 0.1, 1, 10 and 100 MOI Ad SIINFEKL-Luc/5 × 105 DC, the four-hour CTL killing rate were 14.4% ± 2.3%, 32.6% ± 4.5%, 86.7% ± 3.8% and 99.8% ± 0.3%, respectively, and the ratio of IFN-γ-producing CD8+ T cells to total CD8+ T cells in spleen were 0.49% ± 0.12%, 1.74% ± 0.21%, 4.12% ± 0.34% and 9.53% ± 0.58%, respectively. Conclusion: There is an obvious relationship between levels of the antigen expressed by Ad SIINFEKL-Luc and antigen-specific cellular immune responses elicited by the antigen. In a certain range, a positive correlation exists.