The purpose of the experiment was to determine whether or not latent inhibition (the deleterious effect of preconditioning exposure to the CS on subsequent acquisition) is attenuated by interpolating a delay between the last session of preconditioning exposure to the CS and the first session of acquisition. Independent groups of preexposed Ss were given classical eyelid conditioning either immediately following their 550th CS-alone presentation, or 24 h after their 550th CS-alone presentation. Control groups had no experience with the CS prior to acquisition. Preexposed Ss were slower to acquire the CR than were control Ss, replicating previous reports of latent inhibition in the eyelid conditioning situation. Furthermore, the magnitude of latent inhibition was unaffected by the interpolation of a delay interval between preexposure and acquisition.