Development of TIE Procedures for Complex Mixtures Using Physiological Responses in Fish

Methodology was developed for the isolation and recovery of ompounds from a commercial lampricide formulation associated with hepatic mixed function oxygenase (MFO) enzyme induction in fish. MFO induction in laboratory bioassays was characterized and optimized prior to fractionation experiments. Recovery and isolation of MFO activity directed the chemical fractionations. Fractionation techniques were developed on a preparative scale so that …