A novel method for collection and preservation of faeces for genetic studies Journal Articles

abstract

• AbstractQuantitative and qualitative measurements of DNA were used to compare faecal sample storage in ethanol and silica with a novel method (two‐step) in which samples are soaked in ethanol and then desiccated with silica. Silica‐preserved samples had the lowest DNA concentrations. The two‐step method yielded significantly more DNA in high quality samples (average DNA concentrations > 100 pg/µL with all storage methods). However, for lower quality samples, the ethanol and two‐step methods performed similarly. The amounts and rates of sample degradation were not strongly affected by storage method and neither was the percentage of target DNA (< 1%) obtained from the samples.

authors

• ROEDER, AMY D
• ARCHER, FREDERICK I
• Poinar, Hendrik
• MORIN, PHILLIP A

status

• published 

publication date

• December 2004

has subject area

• 06 Biological Sciences (FoR)
• Evolutionary Biology (Science Metrix)

published in

• Molecular Ecology Resources  Journal

keywords

• 31 Biological Sciences
• 3105 Genetics
• Biotechnology
• Genetics

Digital Object Identifier (DOI)

• 10.1111/j.1471-8286.2004.00737.x

start page

• 761

end page

• 764

volume

• 4

issue

• 4