Part F: Directed Differentiation of Human Embryonic Stem Cells into Myeloid Cells

The in vitro aggregation of human embryonic stem (ES) cells into three dimensional clusters, termed embryoid bodies (EB), allows for the spontaneous differentiation of cells representing endoderm, mesoderm and ectoderm lineages. During EB differentiation, the stochastic generation of hematopoietic cells is enhanced by a combination of hematopoietic cytokines and the mesoderm inducer bone morphogenetic protein‐4 (BMP‐4). Under this treatment, up …