Site‐Selective Affinity Labelling of Maltose...

Site‐Selective Affinity Labelling of Maltose Binding Protein in Bacterial Cells

Abstract

Labelling simplified: In two steps, a thioester carbohydrate conjugate can be synthesised that site‐specifically labels maltose binding protein (shown in grey). Specific labelling is observed in crude cell extracts and within live E. coli cells.

Authors

Rullo A; Beharry AA; Gómez‐Biagi RF; Zhao X; Nitz M

Journal

ChemBioChem, Vol. 13, No. 6, pp. 783–787

Publisher

Wiley

Publication Date

April 16, 2012

DOI

10.1002/cbic.201200046

ISSN

1439-4227

Associated Experts

Anthony Rullo

Associate Professor, FHSMED

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