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Selection of Reference Genes for qPCR Analyses of...
Journal article

Selection of Reference Genes for qPCR Analyses of Gene Expression in Ramie Leaves and Roots across Eleven Abiotic/Biotic Treatments

Abstract

Quantitative real-time PCR (qPCR) is commonly used for deciphering gene functions. For effective qPCR analyses, suitable reference genes are needed for normalization. The objective of this study is to identify the appropriate reference gene(s) for qPCR analyses of the leaves and roots of ramie (Boehmeria nivea L.), an important natural fiber crop. To accomplish this goal, we investigated the expression patterns of eight common plant qPCR …

Authors

Yu Y; Zhang G; Chen Y; Bai Q; Gao C; Zeng L; Li Z; Cheng Y; Chen J; Sun X

Journal

Scientific Reports, Vol. 9, No. 1,

Publisher

Springer Nature

DOI

10.1038/s41598-019-56640-3

ISSN

2045-2322