Journal article
Nickel serves as a substrate recognition motif for the endopeptidase involved in hydrogenase maturation
Abstract
The interaction of the hydrogenase maturation endopeptidase HycI with its substrate, the precursor of the large subunit, was studied. Replacement of conserved amino-acid residues in HycI, which have been shown to bind a cadmium ion from the crystallization buffer in crystals of HybD (endopeptidase for hydrogenase 2), abolished or strongly reduced processing activity. Atomic absorption spectroscopy of purified HycI and HybD proteins showed the …
Authors
Theodoratou E; Paschos A; Magalon A; Fritsche E; Huber R; Böck A
Journal
The FEBS Journal, Vol. 267, No. 7, pp. 1995–1999
Publisher
Wiley
Publication Date
April 2000
DOI
10.1046/j.1432-1327.2000.01202.x
ISSN
1742-464X